Alexys Homocysteine in Plasma
Homocysteinaemia is recognised as an independent risk factor for arteriosclerosis and coronary heart disease. Increases of 10-15 % in the level of homocysteine raises the risk of coronary heart disease and cerebral vascular incident by approximately 3 to 4 fold. Homocysteine is an important sulphur containing amino acid in the metabolism of Methionine and Cysteine. With the ALEXYS system a highly sensitive, economical, reproducible and rapid method for the analysis of homocysteine in plasma by isocratic reversed phase LC with electrochemical detection (EC) has been developed. The detection limit with standards is 0.3 nmol/L, RSD in peak heights is better than 1% and homocysteine is eluted within 5 minutes. Recovery from plasma samples is nearly 100%. The chromatogram shows the analysis of homocysteine ([1] 39 µmol/L) and 2MPG ([2] 98 µmol/L) in plasma.
- Direct detection, no derivatisation
- Standardized, fast and reliable assay
- Selective detection on gold electrode
- Robust & reproducible
Configuración del sistema:
Para el análisis de homocisteina se ha seleccionado una celda electroquímica VT-03 con electrodo de trabajo de oro. La celda proporciona una excelente reproducibilidad y selectividad para los grupos tiol.
El Sistema Alexys está compuesto por las siguientes partes:
- Automuestreador refrigerado AS 110
- Bomba LC 110S
- Detector Electroquímico DECADE II, SDC
- Celda VT03 con Au WE, 3 mm ID, ISAAC
- Organizador OR110
- Kit de conexión LC
- Kit de conexión USB, (3 puertos)
- Software de adquisición de datos Clarity
|
Referencia |
Descripción |
|
180.0080C |
ALEXYS Analizador Homocisteina |
