CDT in Serum

CDT (Carbohydrate Deficient Transferrin) is the most specific parameter for chronic alcohol abuse.

Manual Sample preparation.

The ClinRep® HPLC Complete Kit for CDT provides a unique chromatographical resolution compared to other commercially available test kits. The analytical method correlates with the reference method of the IFCC-CDT-WG* and ensures a reliable separation of all relevant transferrin isoforms (a-, mono-, di-, …, hexasialotransferrin). This applies in particular for disialotransferrin, which is the defined analyte by this group. Alternatively to this method with manual sample preparation, ClinRep® Complete Kits for on-line analysis and automated on-line analysis via 96-well plates are available.

*Working group of the IFCC for the international standardisation of CDT measurement.

On-line Analysis.

This ClinRep® Complete Kit is designed for the fast analysis of CDT with on-line SPE HPLC. It features an injection interval of 9.5 min only and provides a unique chromatographical resolution compared to other commercially available test kits. Analogously to our kit with manual sample preparation, the on-line procedure correlates with the candidate reference method of the IFCC-CDT-WG*. Prior to the sample injection, a simple dilution step is required (”sample pre-treatment“, see below). Alternatively, the dilution can be performed automated via 96-well plates. For this purpose an additional ClinRep® Complete Kit is available.

Automated on-line Analysis.

This ClinRep® Complete Kit is designed for the fully automated analysis of CDT with liquid handling systems and subsequent on-line SPE HPLC. The analytical method correlates with the candidate reference method of the IFCC-CDT-WG* and ensures a reliable separation of all relevant transferrin isoforms (a-, mono-, di-, …, hexa-sialotransferrin). This applies in particular for disialotransferrin, which is the defined analyte by this group. An accurate calculation of the CDT value is ensured by baseline separation.

* Working group of the IFCC for the international standardisation of CDT measurement.

Tenemos disponible un manual de usuario para esta aplicación. Puede solicitar enviar un correo electrónico a clinica@ingenieria-analitica.com

Referencia Descripción Cantidad
21100 ClinRep® Kit para CDT transferencia deficiencia en carbohidratos en Suero para 300 análisis 1 pce.
  for 300 assays  
  Contenido:  
  Fase Móvil A 3 x 21007
  Fase Móvil B 1 x 21008
  Fase Móvil C 2 x 21009
  Viales para pretratamiento de muestras con diluyente D 6 x 21120
  Manual  
  Guía Rápida  
  Componentes separados por kit  
21007 Fase Móvil A 2000 ml
21008 Fase Móvil B 2000 ml
21009 Fase Móvil C 2000 ml
21013 Set de caibradores en suero liofilizados (Nivel 1 – 2) 2 x 1 x 1 ml
21014 Solución test 15 ml
21120 Viales para pretratamiento de muestras con diluyente D 50 pcs.
  Accesorios de inicio:  
21030 Columna Analítica con cromatograma test 1 pce.
21032 Soporte de pre-columna. 1 Pre-columna 1 pce.
FK5820 Soporte filtro de acero inoxidable 1 pre-filtro 1 pce.
FK7400 Filtro en línea (acero inoxidable de bajo volumen muerto) 1 pce.
  Servicio relleno:  
21031 Columna Analítica con cromatograma test 1 pce.
  (la columna original se tienen que devolver)  
  Accesorios:  
21033 Pre-columna 5 pcs.
FK1102 Válvula (6-posiciones / 3-canales), incluye driver de control, PEEK 1 pce.
FK5810 Tubo PEEK(conector de pre-filtro a columna) 1 pce.
FK5821 Soporte y repuestos filtros de acero inoxidable  10 pcs.
FK7340 Sellos para la referencia FK7400 4 pcs. each
FW3000 Detector UV-visible completo (460 nm) 1 pce.
RC2002 Solución de hipolclorito sódico (5 %) 12 ml
  ClinChek® Controles:  
21080 Controles en suero liofilizados 10 x 1 ml
  Nivel I  
21081 Controles en suero liofilizados. 10 x 1 ml
  Nivel II  
21082 Controles en suero liofilizados 2 x 5 x 1 ml
  Nivel I, II