Plasma free metanephrines (PFM) are the most specific parameters for the diagnosis of extraadrenal chromaffin tumors. The measurement of PFMs (metanephrine (M), normetanephrine (NM) and 3-methoxytyramine (MT)) is challenging because of their polar nature, their low molecular weight and their low physiological concentration in human plasma.

Manual Sample Preparation.

This ClinMass® Complete Kit represents a reliable and standardised method for the determination of PFM. In contrast to most of the LC-MS/MS methods established in clinical routine analysis, this method does not require dilution or evaporation steps. The measurement of highly concentrated samples is guaranteed. Alternatively to this method with manual sample preparation for standard LC-MS/MS systems, RECIPE offers an LC-MS/MS Complete Kit with on-line SPE analysis.

On-line Analysis.

Most LC-MS/MS methods used in clinical routine analysis involve time- and labour-consuming manual sample preparation for solid phase extraction (SPE). In contrast to this the RECIPE ClinMass® Complete Kit provides a simple sample pretreatment with one-step protein precipitation and subsequent on-line SPE.