CDT in Serum

CDT (Carbohydrate Deficient Transferrin) is the most specific parameter for chronic alcohol abuse.

Manual Sample preparation.

The ClinRep® HPLC Complete Kit for CDT provides a unique chromatographical resolution compared to other commercially available test kits. The analytical method correlates with the reference method of the IFCC-CDT-WG* and ensures a reliable separation of all relevant transferrin isoforms (a-, mono-, di-, …, hexasialotransferrin). This applies in particular for disialotransferrin, which is the defined analyte by this group. Alternatively to this method with manual sample preparation, ClinRep® Complete Kits for on-line analysis and automated on-line analysis via 96-well plates are available.

*Working group of the IFCC for the international standardisation of CDT measurement.

On-line Analysis.

This ClinRep® Complete Kit is designed for the fast analysis of CDT with on-line SPE HPLC. It features an injection interval of 9.5 min only and provides a unique chromatographical resolution compared to other commercially available test kits. Analogously to our kit with manual sample preparation, the on-line procedure correlates with the candidate reference method of the IFCC-CDT-WG*. Prior to the sample injection, a simple dilution step is required (”sample pre-treatment“, see below). Alternatively, the dilution can be performed automated via 96-well plates. For this purpose an additional ClinRep® Complete Kit is available.

Automated on-line Analysis.

This ClinRep® Complete Kit is designed for the fully automated analysis of CDT with liquid handling systems and subsequent on-line SPE HPLC. The analytical method correlates with the candidate reference method of the IFCC-CDT-WG* and ensures a reliable separation of all relevant transferrin isoforms (a-, mono-, di-, …, hexa-sialotransferrin). This applies in particular for disialotransferrin, which is the defined analyte by this group. An accurate calculation of the CDT value is ensured by baseline separation.

* Working group of the IFCC for the international standardisation of CDT measurement.